Knock out of specific maternal vitellogenins in zebrafish (Danio rerio) evokes vital changes in egg proteomic profiles that resemble the phenotype of poor quality eggs

Abstract Background We previously reported the results of CRISPR/Cas9 knock-out (KO) type-I and type-III vitellogenins (Vtgs) in zebrafish, which provided first experimental evidence on essentiality disparate functioning Vtgs at different stages during early development. However, specific contributions types Vtg to major cellular processes remained be investigated. The present study employed liquid chromatography tandem mass spectrometry (LC-MS/MS) meet this deficit. Proteomic profiles zebrafish eggs lacking three simultaneously ( vtg1 -KO), or only type III vtg3 -KO) were compared those wild (Wt) eggs. Obtained spectra searched against a proteome database identified proteins quantified based normalized spectral counts. Results vtg -KO caused severe changes 1-cell stage These disclosed by molecular signatures that highly resembled proteomic phenotype poor quality our prior studies. perturbations abundances hundreds revealed unique, noncompensable multiple protein energy homeostasis. lack contribution appears have significant impact endoplasmic reticulum mitochondrial functions, thus embryonic development, even after zygotic genome activation. Increased stress, Redox/Detox activities, glycolysis/gluconeogenesis, enrichment proliferation human neurodegenerative disease related activities both - found indicators aforementioned conditions. Distinctive increase apoptosis Parkinson pathways, as well decrease lipid metabolism implies compelling roles Vtg3, least abundant form vertebrate eggs, activities. Several differentially representing altered mechanisms been strong candidate markers for studying details these development possibly other vertebrates. Conclusions findings indicate global egg is subject extensive modification depending presence absence modifications can developmental competence.